Test Details
Methodology
Fluorescence in situ hybridization (FISH) and plasma cell enrichment
Result Turnaround Time
5 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Fluorescence in situ hybridization (FISH) for multiple myeloma (MM), targeting 13q14, IGH/CCND1, TP53, FGFR1/IGH, cMAF/IGH, amplification of 1p/1q, on plasma enriched cells.
Use
This is a diagnostic test for multiple myeloma. Plasma cell enrichment diagnosis increased as much as 50% to 100%. The FISH results on the enriched assay should not be used as a quantitative assay, since the abnormal cells do not represent the percentage of abnormal cells in the aspirate.
Special Instructions
Indicate pertinent clinical diagnosis and previous cytogenetics studies on the test request form. If insufficient plasma cells are available, the following probe priority will be followed: Previous abnormality, p53, FGFR3/IGH, MAF/IGH, 1p/1q, 13q14, CCND1/IGH, 7/9/15 aneuploidy probes. If a chromosome study (510999) is ordered concurrently and sample is not sufficient for both tests, FISH will be prioritized.
Limitations
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Molecular mutations This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. |
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration. |
References
Specimen Requirements
Specimen
Bone marrow
Volume
1 to 3 mL bone marrow
Minimum Volume
1 mL bone marrow
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavendar-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.
Green-top (sodium heparin) tube |
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavendar-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity. |
Collection Instructions
Utilize sterile technique and Labcorp transport kits when possible.
|
Utilize sterile technique and Labcorp transport kits when possible. |
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Whole blood; broken Vacutainer®; specimen received at testing facility 72 hours after collection
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510325 | Multiple Myeloma FISH Panel | 510768 | Specimen Type | 31208-2 | ||
| 510325 | Multiple Myeloma FISH Panel | 510695 | Cells Counted | 62361-1 | ||
| 510325 | Multiple Myeloma FISH Panel | 510696 | Cells Analyzed | 62360-3 | ||
| 510325 | Multiple Myeloma FISH Panel | 510769 | FISH Result | 62357-9 | ||
| 510325 | Multiple Myeloma FISH Panel | 510318 | Interpretation | 62365-2 | ||
| 510325 | Multiple Myeloma FISH Panel | 510319 | Director Review: | 48672-0 | ||
| 510325 | Multiple Myeloma FISH Panel | 512123 | 11502-2 | |||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510768 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510695 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510696 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510769 | |||||
| Result Code Name | FISH Result | |||||
| UofM | ||||||
| Result LOINC | 62357-9 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510318 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 62365-2 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 510319 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 | |||||
| Order Code | 510325 | |||||
| Order Code Name | Multiple Myeloma FISH Panel | |||||
| Order Loinc | ||||||
| Result Code | 512123 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 |